Leaf-colonising Bacteria Colony Phenotype

May 22, 2020 22:59 · 400 words · 2 minute read Bacteria

Here are some bacteria I am working with. I will try to update some interesting info about these bacteria.

These bacteria were subcultured on R2A agar.

Phylogenetic tree

Retrived from my MSc thesis

Here is a picture showing brief pigment and colony phenotype.

Full names will be listed below respectively.

Genus Sphingomonas

Sphingomonas phyllosphaerae FA2

Sphingomonas melonis Fr1


Wild type S. melonis

Due to the effect of mScarlt fluorescence, the pigment of bacteria colonies is also changed.


mScarlet fluoscent S. melonis

Sphingomonas sp. leaf 34

Sphingomonas sp. leaf 17

Sphingomonas sp. leaf 357

Genus Methylobacterium

Methylobacterium sp. leaf 85

Methylobacterium radiotolerans sp. 0-1

Methylobacterium sp. leaf 92


Wild type M. sp. leaf 92

mScarlet fluoscent M. sp. leaf 92

Acidovorax sp. leaf 84


Wild type A.sp. leaf 84

The pigment for fluorescent A. sp. leaf 84 does not change.


mScarlet fluoscent A.sp. leaf 84

Genus Pseudomonas

Pseudomonas syringae B728a

Pseudomonas syringae B728a is a bean pathogen and it was claimed that it is non-pathogenic to Arabidopsis thaliana (Vinatzer et al. 2006). However, in my experiment, it does show a negative effect on Arabidopsis growth.

Pseudomonas citronellolis


Wild type P. citronellolis

For P. cironellolis, now it is little pinkish.


mScarlet fluoscent P. citronellolis

Pseudomonas koreensis

Pantoea agglomerans 299R


Wild type P. agglomerans

However, the pigment turns orange for genus Pantoea.


mScarlet fluoscent P. agglomerans

Pantoea vegans C9-1

Pantoea vegans PW

Pantoea vagans PW is a white variant of C9-1 and misses a mega plasmid.

Williamsia sp. leaf 354

Rhodococcus leaf 225

Aeromicrobium sp. leaf 245

Arthrobacter sp. leaf 145

Microbacterium sp. leaf 320

Microbacterium sp. leaf 347

Plantibacter sp. leaf 1

Rathayibacter sp. leaf 296

Agreia sp. leaf 335

Erwinia amylovora

Bradyrhizobium sp. leaf 396

Pedobacter sp. leaf 194

Xylophilus sp. leaf 220

All fluorescent bacteria are construted by dear Rudolf (2018)

Reference

Schlechter, Rudolf O., Hyunwoo Jun, Michał Bernach, Simisola Oso, Erica Boyd, Dian A. Muñoz-Lintz, Renwick C. J. Dobson, Daniela M. Remus, and Mitja N. P. Remus-Emsermann. 2018. “Chromatic Bacteria - A Broad Host-Range Plasmid and Chromosomal Insertion Toolbox for Fluorescent Protein Expression in Bacteria.” Frontiers in Microbiology 9 (December): 3052.

Vinatzer, B. A., Teitzel, G. M., Lee, M.-W., Jelenska, J., Hotton, S., Fairfax, K., Jenrette, J., & Greenberg, J. T. (2006). The type III effector repertoire of Pseudomonas syringae pv. syringae B728a and its role in survival and disease on host and non-host plants. Molecular Microbiology, 62(1), 26–44.

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